Laboratori ea liteko ea lekala la Federal State Budgetary Institution "Rosselkhoztsentr" sebakeng sa Krasnoyarsk e qalile mofuta o mocha oa lipatlisiso - ho lemoha le ho khetholla likokoana-hloko tse fetotsoeng ka liphatsa tsa lefutso (GMOs) ho sebelisa mokhoa oa polymerase chain reaction ka nako ea sebele.
Sebakeng sa Federation ea Russia, boteng ba li-GMO lijong le lijo-thollong tsa lijo bo laoloa ke Melao ea Tekheniki ea Customs Union TR CU 015/2011 "Ka polokeho ea lijo-thollo"; ka hona, taolo ea litaba tsa GMO lijo-thollo. e tlamehile. Hape, ho ea ka molao oa tlhahiso ea peo (Molao oa Federal No. 17.12.1997-FZ oa la 149 December, 11, joalokaha o fetotsoe ka la 2021 June, XNUMX), sebakeng sa Russia Federation ho thibetsoe ho sebelisa ho jala (ho lema) peo. ea limela tseo lenaneo la tsona la liphatsa tsa lefutso le fetotsoeng ho sebelisoa mekhoa ea liphatsa tsa lefutso. Hona joale, molao o mocha "Ka tlhahiso ea peo" No. 454-FZ e amohetsoe (e qala ho sebetsa ka la 1 September 2023), ho latela hore na lisebelisoa tsohle tsa peo li lokela ho lekoa bakeng sa litaba tsa GMO.
Sebōpuoa se fetotsoeng ka liphatsa tsa lefutso ke ntho e phelang eo genotype ea eona e fetotsoeng ka mokhoa oa maiketsetso ho sebelisoa mekhoa ea boenjiniere ba liphatsa tsa lefutso e le hore e fane ka thepa e lakatsehang. Temong, boenjiniere ba liphatsa tsa lefutso bo sebelisoa ho theha mefuta e mecha ea limela tse hanyetsanang le maemo a sa thabiseng a tikoloho le likokoanyana, 'me li na le litšobotsi tse ntle tsa kholo le tatso.
Mokhoa o nepahetseng ka ho fetesisa oa ho bona li-GMO lihlahisoa tsa lijo le lijo-thollo ke mokhoa oa 'nete oa nako ea polymerase chain reaction (PCR). Ona ke mokhoa o hlokolosi haholo le o nepahetseng haholo o ipapisitseng le ho kopitsoa khafetsa ho khetha karolo e itseng ea DNA nucleic acid ho sebelisa li-enzyme tlas'a maemo a maiketsetso (in vitro). PCR e u lumella ho fumana karolo e nyane ea tlhaiso-leseling ea liphatsa tsa lefutso ka har'a lintho tse ithutoang ka tatellano e itseng ea DNA nucleotide ea ntho efe kapa efe har'a palo e kholo ea likarolo tse ling tsa DNA le ho e atisa ka makhetlo a mangata.
Ka laboratoring ea liteko ea lekala la Federal State Budgetary Institution "Rosselkhoztsentr" sebakeng sa Krasnoyarsk, mosebetsi oa ho ntša DNA ho tloha lihlahisoa tsa semela le ho etsa PCR o etsoa ka lisebelisoa tsa morao-rao tse finyellang litlhoko tsa ho hlophisa laboratori ea PCR. Amplification (sebopeho sa likopi tse ngata tsa likarolo tsa chromosomal DNA) se etsoa ho sebelisa amplifier ea ANK-M, e kenyellelitsoeng ho ngolisa lisebelisoa tsa ho lekanya tsa tsamaiso ea Arshin FSIS. Sesebelisoa sena se na le thermoblock e bolokang mocheso o hlokahalang bakeng sa karabelo mme e hlophisitsoe ho sebelisoa microcomputer e hahelletsoeng, 'me e na le sesebelisoa sa fluorescence se rekotang lets'oao la fluorescent ho tsoa motsoakong oa karabelo ho rera ho bokella sehlahisoa sa PCR.
PCR e etsoa ho sebelisoa lisebelisoa tsa reagent tse nang le ts'ebetso e phahameng (ho feta 90%) ea karabelo ea PCR, kutlo e phahameng le ho hlaka bakeng sa ho sibolloa ha li-GMO, ho fihlela litlhoko tsa machabeng bakeng sa lipehelo tsa moeli oa tlhahlobo (LOD) le moeli oa palo. (LOQ), e nang le taolo e ntle ea kahare (VPK), e lumellang ho qhelela ka thoko liphetho tse mpe tse fosahetseng.
Mokhahlelo oa pele oa tlhahlobo ea ho khetholla li-GMO ke tlhahlobo ea GMO - thuto e felletseng ea ho fumana DNA ea semela le ho tseba likarolo tse itseng tsa lefutso - tatelano e itseng ea taolo (li-promoters le terminators) le liphatsa tsa lefutso. Haeba e 'ngoe ea likarolo tsena e fumanoa, ho tsejoa mela e fetotsoeng ka liphatsa tsa lefutso tsa mefuta e itseng ea limela.
Ka laboratoring ea teko ea lekala la Federal State Budgetary Institution "Rosselkhoztsentr" sebakeng sa Krasnoyarsk Territory, ho ntse ho etsoa liphuputso ho khetholla li-GMO lijong le lijong tsa lijo-thollo le ka peo ea temo. Mosebetsi o etsoa ke litsebi tse koetlisitsoeng ka sehlooho se reng "Metheo ea ho khetholla li-GMO ho lihlahisoa tsa limela tse sebelisang PCR ea nako ea sebele" Setsing sa Federal State Budgetary Institution "Federal Center for Assessment of Safety and Quality of Grain and Its". Ho sebetsa lihlahisoa" sebakeng sa Moscow.
Kahoo, litsebi tsa laboratori ea liteko li atlehile ho tseba mokhoa o mocha, oa sejoale-joale ebile o ts'episa oa ho sekaseka lisebelisoa tsa semela bakeng sa litaba tsa GMO. Nakong e tlang, ho reriloe ho kenyelletsa mokhoa ona sebakeng sa tumello ea lilaboratori tsa liteko ka tsamaiso ea Kamohelo ea Russia.